Počet záznamů: 1
Revelation of fibroblast protein commonalities and differences and their possible roles in wound healing and tumourigenesis using co-culture models of cells
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SYSNO ASEP 0429766 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Revelation of fibroblast protein commonalities and differences and their possible roles in wound healing and tumourigenesis using co-culture models of cells Tvůrce(i) Jarkovská, Karla (UZFG-Y) RID
Dvořánková, B. (CZ)
Halada, Petr (MBU-M) RID, ORCID
Kodet, O. (CZ)
Szabo, P. (CZ)
Gadher, S. J. (US)
Motlík, Jan (UZFG-Y) RID, ORCID
Kovářová, Hana (UZFG-Y) RID, ORCID
Smetana, K. (CZ)Zdroj.dok. Biology of the Cell. - : Wiley - ISSN 0248-4900
Roč. 106, č. 7 (2014), s. 203-218Poč.str. 16 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. FR - Francie Klíč. slova cancer-associated fibroblasts ; contractile proteins ; myofibroblasts ; tissue injury ; tumourigeneses Vědní obor RIV FD - Onkologie a hematologie CEP ED2.1.00/03.0124 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UZFG-Y - RVO:67985904 ; MBU-M - RVO:61388971 UT WOS 000339013600001 EID SCOPUS 84903512542 DOI 10.1111/boc.201400014 Anotace Background information: The in vitro co-culture models of communication between normal fibroblasts and epithelial cells, such as keratinocytes or squamous cell carcinoma cells of FaDu line representing wound healing or cancer development, were established by non-direct contact between the cells and utilised in this study to examine epithelia-induced changes in overall fibroblast proteome patterns. Results: We were able to select the proteins co-regulated in both models in order to evaluate possible molecular commonalities between wound healing and tumour development. Amongst the most pronounced were the proteins implemented in contractile activity and formation of actin cytoskeleton such as caldesmon, calponin-2, myosin regulatory light-chain 12A and cofilin-1, which were expressed independently of the presence of α-smooth muscle actin. Additionally, proteins altered differently highlighted functional and cellular phenotypes during transition of fibroblasts towards myofibroblasts or cancer-associated fibroblasts. Results showed coordinated regulation of cytoskeleton proteins selective for wound healing which were lost in tumourigenesis model. Vimentin bridged this group of proteins with other regulated proteins in human fibroblasts involved in protein or RNA processing and metabolic regulation. Conclusions: The findings provide strong support for crucial role of stromal microenvironment in wound healing and tumourigenesis. In particular, epithelia-induced protein changes in fibroblasts offer new potential targets which may lead to novel tailored cancer therapeutic strategies. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2015
Počet záznamů: 1