Počet záznamů: 1
Expression of estrogen receptor beta (ERβ) In murine male reproductive tract and sperm
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SYSNO ASEP 0428720 Druh ASEP K - Konferenční příspěvek (lokální konf.) Zařazení RIV Záznam nebyl označen do RIV Název Expression of estrogen receptor beta (ERβ) In murine male reproductive tract and sperm Tvůrce(i) Dostálová, Pavla (BTO-N)
Děd, Lukáš (BTO-N) RID
Dorosh, Andriy (BTO-N) RID
Elzeinová, Fatima (BTO-N) RID
Pěknicová, Jana (BTO-N) RIDCelkový počet autorů 5 Zdroj.dok. Book of abstracts XXth Symposium of Biology and Immunology of Reproduction with international participation. - Praha : Biotechnologický ústav AVČR, v.v.i, 2014 / Pěknicová Jana
S. 1-82Poč.str. 2 s. Akce XXth Symposium of Biology and Immunology of Reproduction with international participation Datum konání 22.05.2014-24.05.2014 Místo konání Třešť Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova estrogene receptor beta ; spermatozoa ; splice variant Vědní obor RIV CE - Biochemie CEP 14-055547S GA ČR - Grantová agentura ČR CZ1.05/1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BTO-N - RVO:86652036 CEZ AV0Z50520701 - BTO-N (2007-2013) Anotace Estrogens are steroid hormones that play an important role in reproduction of both sexes. In male, the main source of estrogens are testes where both somatic and germ cells are responsible for testosteron conversion to estrogens. Estrogens are involved in control of spermatogenesis, fluid reabsorption in rete testis and epididymis, and in later maturation steps that sperm undergo in female genital tract (capacitation, acrosome reaction). Generally, estrogen action is mediated through binding to estrogen receptors (ERs) which than lead to classical genomic or rapid non-genomic signaling. Nowadays, two classical estrogen receptors are known – ERα and ERβ. ERβ is a predominant variant in testes, while ERα is more abundant in rete testis and initial segment of epididymis. In addition to classical ERs, several splice variants that can differ in their ligand- or DNA-binding properties were detected in different tissues and cell lines. ERs mostly work as a dimer (homo- and hetero-) and splice variants often „only“ modulate function of classical full-length ERs. Therefore, estrogen action seems to be a very complex. To contribute to understanding of estrogen action in male, we detected ERβ and its potential splice variants in mice testis, epididymis and sperm. According to our results, two variants are present in all analysed tissues and cells. These variants differ in one exon in ligand binding domain which leads to different affinity for estrogens. To analyse these variants also at a protein level, we prepared specific monoclonal antibodies recognizing particular variant of ERβ. Both atibodies detected band(s) in protein extracts from testes or epididymis. Taking together, there are at least two variants of ERβ in mice testes, epididymis and sperm and it seems that both variants are similar in abundance within the same organ or sperm. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2015
Počet záznamů: 1