Počet záznamů: 1
Prenatal exposure to endocrine disruptors induces trans-generational deregulation of microRNAs involved in the LIN28-LET-7-BLIMP1 pathway in male primordial germ cells
- 1.
SYSNO ASEP 0428098 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název Prenatal exposure to endocrine disruptors induces trans-generational deregulation of microRNAs involved in the LIN28-LET-7-BLIMP1 pathway in male primordial germ cells Tvůrce(i) Brieño-Enríquez, M.A. (ES)
López, J.G. (ES)
Cárdenas, D.B. (ES)
Guibert, S. (FR)
Děd, Lukáš (BTO-N) RID
Cleroux, E. (FR)
Hourcade, J.D. (ES)
Pěknicová, Jana (BTO-N) RID
Weber, M. (FR)
del Mazo, J. (ES)Celkový počet autorů 10 Zdroj.dok. Abstracts of papers presented at the 2014 Cold Spring Harbor Asia Conference. - Suzhou : Cold Spring Harbor Laboratory, 2014
S. 150-150Poč.str. 1 s. Akce Epigenetics, chromatin and transcription Datum konání 05.05.2014-09.05.2014 Místo konání Suzhou Země CN - Čína Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CN - Čína Klíč. slova primordial germ cells ; microRNA ; endocrine disruptor ; methylation Vědní obor RIV EB - Genetika a molekulární biologie CEP GAP503/12/1834 GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BTO-N - RVO:86652036 Anotace Primordial germ cells (PGC) are the embryonic precursors of the germ cell linage, which are restricted to form only sperm and oocytes following their specification from pluripotent cells. PGC precursors are specified in the epiblast around 6.25 days post coitum (dpc), and around 7.25 dpc become identifiable in a 40 cell-cluster. Thereafter, PGC migrates through hindgut endoderm and colonize the genital ridges at day 10.5. PGC specification depends on interactions among various molecular factors including different microRNA and microRNA-regulated molecules. In the present study, we used a mouse model to evaluate the trans-generational (F1-F3) effects of endocrine disruptor with anti-androgenic effects vinclozolin (VCZ) administrated in two doses. We observed decreased fertility rate, higher apoptotic rate and histopathologic alterations in adult testis, PGC number reduction, increments of PGC apoptosis and changes in PGC gene expression. In the attempt to clarify the trans-generational transmition of the altered phenotypes, we performed the microRNA expression and DNA methylation analysis. We observed the significant alteration in the expression of multiple microRNA and microRNA-regulated genes which are important for PGCs specification, including LIN28, let-7 and BLIMP1. In the absence of microRNA-binding protein LIN28, microRNA let-7 binds to the 3´UTR of the Blimp1 mRNA to block its translation and prevent PGCs from developing. LIN28 binds to the let-7 family pri-miRNA loop to prevent processing of these precursor forms into the mature let-7 miRNA, allowing BLIMP1 translation, and permitting PGC specification. Therefore, trans-generational deregulation in the expression of factors involved in the Lin28-let-7-Blimp1 pathway can lead to specific VCZ-induced phenotype observed in our study. The possible mechanisms responsible for the trans-generational transmission of altered microRNA expression patterns can involve paramutations and/or altered DNA methylation. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2015
Počet záznamů: 1