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Magnetically modified spent grain as a low-cost, biocompatible and smart carrier for enzyme immobilisation
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SYSNO ASEP 0398937 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Magnetically modified spent grain as a low-cost, biocompatible and smart carrier for enzyme immobilisation Tvůrce(i) Pospišková, K. (CZ)
Šafařík, Ivo (UEK-B) RID, SAIZdroj.dok. Journal of the Science of Food and Agriculture. - : Wiley - ISSN 0022-5142
Roč. 93, č. 7 (2013), s. 1598-1602Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova magnetic fluid ; spent grain ; lipase ; magnetic carrier ; immobilisation Vědní obor RIV EI - Biotechnologie a bionika CEP GAP503/11/2263 GA ČR - Grantová agentura ČR LH12190 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora RVO:67179843 - RVO:67179843 UT WOS 000317614000010 DOI 10.1002/jsfa.5930 Anotace BACKGROUND: Food and feed technology and biotechnology benefit from the use of immobilised enzymes. New low-cost enzyme carriers exhibiting high biocompatibility and response to external magnetic field can substantially improve the application potential of immobilised enzyme systems. RESULTS: Ferrofluid-modified spent grain was used as a low-cost, biocompatible and magnetically responsive carrier for the immobilisation of Candida rugosa lipase. Several immobilisation procedures were tested using both native and poly(ethyleneimine)-modified magnetic spent grain. Activity of immobilised lipase per unit mass of carrier, operational stability, time stability and Michaelis constant were compared. In general, magnetic spent grain modified with poly(ethyleneimine) bound a smaller amount of active lipase than unmodified magnetic spent grain, but the operational and storage stabilities of enzyme immobilised on poly(ethyleneimine)-modified carrier were very high. CONCLUSION: Ferrofluid-modified spent grain can be a promising low-cost magnetic carrier for enzyme immobilisation, applicable e.g. in food and feed technology and biotechnology. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2014
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