Počet záznamů: 1
Nucleobase protection strategy for gene cloning and expression
- 1.0393858 - ÚOCHB 2014 RIV DE eng J - Článek v odborném periodiku
Kielkowski, Pavel - Brock, N. L. - Dickschat, J. S. - Hocek, Michal
Nucleobase protection strategy for gene cloning and expression.
Chembiochem. Roč. 14, č. 7 (2013), s. 801-804. ISSN 1439-4227. E-ISSN 1439-7633
Grant CEP: GA ČR GA203/09/0317
Institucionální podpora: RVO:61388963
Klíčová slova: DNA * polymerase chain reaction * protecting groups * protein production * restriction endonucleases
Kód oboru RIV: CE - Biochemie
Impakt faktor: 3.060, rok: 2013
Protecting group chemistry meets molecular biology: Chemically modified dATP carrying a bulky triethylsilylethynyl group was used in a PCR-based synthesis of a gene internally protected against cleavage by restriction endonucleases. The unmodified flanking regions were cleaved for cloning into a plasmid which was replicated by E. coli, and used for protein production.
Trvalý link: http://hdl.handle.net/11104/0222228
Počet záznamů: 1