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The key role of insulin-like growth factor I in limbal stem cell differentiation and the corneal wound-healing process
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SYSNO ASEP 0392142 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The key role of insulin-like growth factor I in limbal stem cell differentiation and the corneal wound-healing process Tvůrce(i) Trošan, Peter (UMG-J)
Svobodová, Eliška (UMG-J)
Chudíčková, Milada (UMG-J)
Krulová, Magdalena (UMG-J)
Zajícová, Alena (UMG-J)
Holáň, Vladimír (UMG-J)Zdroj.dok. Stem Cells and Development. - : Mary Ann Liebert - ISSN 1547-3287
Roč. 21, č. 18 (2012), s. 3341-3350Poč.str. 10 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova IGF-I ; limbal stem cells ; cornea ; healing Vědní obor RIV EB - Genetika a molekulární biologie CEP GAP304/11/0653 GA ČR - Grantová agentura ČR GAP301/11/1568 GA ČR - Grantová agentura ČR GD310/08/H077 GA ČR - Grantová agentura ČR KAN200520804 GA AV ČR - Akademie věd Institucionální podpora UMG-J - RVO:68378050 UT WOS 000311953700010 DOI 10.1089/scd.2012.0180 Anotace Limbal stem cells (LSC), which reside in the basal layer of the limbus, are thought to be responsible for corneal epithelial healing after injury. When the cornea is damaged, LSC start to proliferate, differentiate, and migrate to the site of injury. To characterize the signaling molecules ensuring communication between the cornea and LSC, we established a mouse model of mechanical corneal damage. The central cornea or limbal tissue was excised at different time intervals after injury, and the expression of genes in the explants was determined. It was observed that a number of genes for growth and differentiation factors were significantly upregulated in the cornea rapidly after injury. The ability of these factors to regulate the differentiation and proliferation of limbal cells was tested. It was found that the insulin-like growth factor-I (IGF-I), which is rapidly overexpressed after injury, enhances the expression of IGF receptor in limbal cells and induces the differentiation of LSC into cells expressing the corneal cell marker, cytokeratin K12, without any effect on limbal cell proliferation. In contrast, the epidermal growth factor (EGF) and fibroblast growth factor-beta (FGF-beta), which are also produced by the damaged corneal epithelium, supported limbal cell proliferation without any effect on their differentiation. Other factors did not affect limbal cell differentiation or proliferation. Thus, IGF-I was identified as the main factor stimulating the expression of IGF receptors in limbal cells and inducing the differentiation of LSC into cells expressing corneal epithelial cell markers. The proliferation of these cells was supported by EGF and FGF. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2014
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