Počet záznamů: 1
Genome-wide identification of genes directly regulated by the pleiotropic transcription factor Spx in Bacillus subtilis
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SYSNO ASEP 0387080 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Genome-wide identification of genes directly regulated by the pleiotropic transcription factor Spx in Bacillus subtilis Tvůrce(i) Rochat, T. (FR)
Nicolas, P. (FR)
Delumeau, O. (FR)
Rabatinová, Alžběta (MBU-M)
Korelusová, Jana (MBU-M)
Leduc, A. (FR)
Bessieres, P. (FR)
Dervyn, E. (FR)
Krásný, Libor (MBU-M) RID, ORCID
Noirot, P. (FR)Zdroj.dok. Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048
Roč. 40, č. 19 (2012), s. 9571-9583Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova C-TERMINAL DOMAIN ; RNA-POLYMERASE ; DISULFIDE STRESS Vědní obor RIV CE - Biochemie CEP GAP302/11/0855 GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000310377200026 DOI https://doi.org/10.1093/nar/gks755 Anotace The transcriptional regulator Spx plays a key role in maintaining the redox homeostasis of Bacillus subtilis cells exposed to disulfide stress. Defects in Spx were previously shown to lead to differential expression of numerous genes but direct and indirect regulatory effects could not be distinguished. Here we identified 283 discrete chromosomal sites potentially bound by the Spx-RNA polymerase (Spx-RNAP) complex using chromatin immunoprecipitation of Spx. Three quarters of these sites were located near Sigma(A)-dependent promoters, and upon diamide treatment, the fraction of the Spx-RNAP complex increased in parallel with the number and occupancy of DNA sites. Correlation of Spx-RNAP-binding sites with gene differential expression in wild-type and delta spx strains exposed or not to diamide revealed that 144 transcription units comprising 275 genes were potentially under direct Spx regulation. Spx-controlled promoters exhibited an extended -35 box in which nucleotide composition at the -43/-44 positions strongly correlated with observed activation. In vitro transcription confirmed activation by oxidized Spx of seven newly identified promoters, of which one was also activated by reduced Spx. Our study globally characterized the Spx regulatory network, revealing its role in the basal expression of some genes and its complex interplay with other stress responses Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2013
Počet záznamů: 1