Počet záznamů: 1
Fine structure and morphology of sterlet (Acipenser ruthenus L. 1758) spermatozoa and acrosin localization
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SYSNO ASEP 0381935 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Fine structure and morphology of sterlet (Acipenser ruthenus L. 1758) spermatozoa and acrosin localization Tvůrce(i) Pšenička, M. (CZ)
Vančová, M. (CZ)
Koubek, Pavel (BTO-N)
Těšitel, J. (CZ)
Linhart, O. (CZ)Celkový počet autorů 5 Zdroj.dok. Animal Reproduction Science. - : Elsevier - ISSN 0378-4320
Roč. 111, č. 1 (2009), s. 3-16Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Acipenser ruthenus ; Acrosome ; Acrosin ; Morphometric analysis ; SEM ; TEM Vědní obor RIV EG - Zoologie CEP GA524/06/0817 GA ČR - Grantová agentura ČR CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000262681600002 DOI 10.1016/j.anireprosci.2008.02.006 Anotace Ultrastructure of sterlet Acipenser ruthenus L. 1758 sperm was examined by scanning and transmission electron microscopy, which allowed us to use various methods for visualizations of different parts of sterlet spermatozoa. Sperm cells possess a head with a distinct acrosome, a midpiece and a single flagellum surrounded by the flagellar plasma membrane. The average length of the head including the acrosome and the midpiece was estimated as 5.14 +/- 0.42 mu m. Nine to 10 posterolateral projections were derived from the acrosome. Three inter-twining endonuclear canals bounded by membranes traversed the nucleus in its whole length from the acrosome to the implantation fossa. Acrosin was located in all the three parts (acrosome, endonuclear canals and implantation fossa). The proximal and distal centrioles located in the midpiece compacted of nine peripheral triplets of microtubules. One cut of the midpiece contained from two to six mitochondria with area of 215 +/- 85 nm(2) in average. The flagellum was 42.47 +/- 1.89 mu m in length with typical eukaryotic organization of one central pair and nine peripheral pairs of microtubules. It passed through a cytoplasmic channel in the midpiece, which was formed by an invagination at the plasmallemma. The flagellum gradually developed two lateral extensions of its plasma membrane, so-called "fins". Detected morphological variation can be described by four principal component axes corresponding to groups of individual morphometric characters defined on the sperm structures. Correlations among the characters indicate that the sperms are variable in their shape rather than size. Significant variation among examined fish individuals was found only in flagellum and nucleus length. Comparison between the present and previous studies of morphology of sturgeon spermatozoa confirmed large inter- and/or intra-specific differences that could be of substantial taxonomic value. (C) 2008 Elsevier B.V. All rights reserved. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2013
Počet záznamů: 1