Počet záznamů: 1
Metabolic activity and membrane integrity changes in Microcystis aeruginosa – new findings on hydrogen peroxide toxicity in cyanobacteria
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SYSNO ASEP 0379236 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Metabolic activity and membrane integrity changes in Microcystis aeruginosa – new findings on hydrogen peroxide toxicity in cyanobacteria Tvůrce(i) Mikula, Přemysl (BU-J)
Zezulka, Štěpán (BU-J) ORCID
Jančula, Daniel (BU-J) RID
Maršálek, Blahoslav (BU-J) ORCID, SAICelkový počet autorů 4 Zdroj.dok. European Journal of Phycology. - : Taylor & Francis - ISSN 0967-0262
Roč. 47, č. 3 (2012), s. 195-206Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova hydrogen peroxide ; cyanobacteria ; flow cytometry Vědní obor RIV EF - Botanika CEP FR-TI3/196 GA MPO - Ministerstvo průmyslu a obchodu TA01010356 GA TA ČR - Technologická agentura ČR Institucionální podpora BU-J - RVO:67985939 UT WOS 000308435700001 DOI 10.1080/09670262.2012.687144 Anotace The aim of our study was to investigate the intracellular toxicity mechanisms of potentially anti-cyanobacterial agent hydrogen peroxide in Microcystis aeruginosa. Metabolic activity and cell membrane integrity were evaluated by flow cytometry in cyanobacteria exposed to the substance tested in the dark or light; the relationships between exposure effects and the kinetics of hydrogen peroxide decomposition were studied. Flow cytometry and chlorophyll a fluorescence measurements suggested that hydrogen peroxide exposure elicits an immediate decline of metabolic activity, and immediate changes of chlorophyll a fluorescence parameters, followed later by an increase in the percentage of membrane-compromised cells. When the concentration of hydrogen peroxide used was lethal, a significant drop in total cell counts was detected. Our study also confirmed that light is the critical factor affecting peroxide decomposition and thus greatly influences its toxicity. Pracoviště Botanický ústav Kontakt Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Rok sběru 2013
Počet záznamů: 1