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Cadmium-induced production of phytochelatins and speciation of intracellular cadmium in organs of Linum usitatissimum seedlings
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SYSNO ASEP 0378909 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cadmium-induced production of phytochelatins and speciation of intracellular cadmium in organs of Linum usitatissimum seedlings Tvůrce(i) Najmanová, J. (CZ)
Neumannová, E. (CZ)
Leonhardt, T. (CZ)
Zítka, O. (CZ)
Kižek, R. (CZ)
Macek, Tomáš (UOCHB-X)
Macková, M. (CZ)
Kotrba, P. (CZ)Celkový počet autorů 8 Zdroj.dok. Industrial Crops and Products. - : Elsevier - ISSN 0926-6690
Roč. 36, č. 1 (2012), s. 536-542Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova flax ; cadmium ; heavy metal tolerance ; phytochelatins ; phytoremediation Vědní obor RIV EI - Biotechnologie a bionika CEP 1M06030 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000300921100078 DOI 10.1016/j.indcrop.2011.11.008 Anotace The capacity of most flax (Linum usitatissimum L) varieties and cultivars to accumulate cadmium (Cd), underlined by their Cd-tolerant phenotype, brought an interest in their use in phytoextraction of soil Cd to aboveground biomass. The aim of this study was to gain an insight into the mechanism of intracellular detoxification of Cd, by exploring the response of flax seedlings, cultivar ACT 952, to 12 days growth at 50-500 mu M Cd. The majority of accumulated Cd (67-74%) was retained in roots, which showed reduced elongation (by 50% at 100 mu M Cd) and 3.5 (at 50 mu M Cd) to 9.6 (at 500 mu M Cd) times higher concentration of Cd than in shoots. Labeling of roots with Leadmium fluorescent marker visualized intracellular localization of Cd. High-performance liquid chromatography of extracts from Cd-treated seedlings revealed induced synthesis of phytochelatins, small intracellular peptides binding Cd through cysteine of their repeating (gamma-Glu-Cys)(n) motives (PCn). Regardless the treatment, PC3 was dominant in all organs and PC2 was restricted to cotyledons. Larger PC4 and PC5 accumulated only in roots grown at 500 mu M Cd. Size exclusion chromatography of the organ extracts from seedlings exposed to 100 mu M revealed the presence of 2-kDa complexes equated to low-molecular weight (LMW) Cd-PC complexes. In roots, the majority of Cd was stored in >= 9-kDa complex corresponding in apparent molecular mass to stable high-molecular weight (HMW) CdS-PC complexes of yeasts and plants. Data demonstrate that, like in most other plants, the response of flax to Cd involved phytochelatins and suggested that the deposition of Cd in HMW complex should be considered the intrinsic feature reducing translocation of Cd to shoots. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2013
Počet záznamů: 1