Počet záznamů: 1
Influence of water environment on biological specimens imaging in ESEM
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SYSNO ASEP 0205651 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název Influence of water environment on biological specimens imaging in ESEM Tvůrce(i) Autrata, Rudolf (UPT-D)
Neděla, Vilém (UPT-D) RID, ORCID, SAI
Horký, D. (CZ)Zdroj.dok. Proceedings of the 6th Multinational Congress on Microscopy - European Extension / Milat O. ; Ježek D.. - Zagreb : Croatian Society for Electron Microscopy, 2003 Rozsah stran s. 497 - 498 Poč.str. 2 s. Akce MCEM Datum konání 01.06.2003-05.06.2003 Místo konání Pula Země HR - Chorvatsko Typ akce EUR Jazyk dok. eng - angličtina Země vyd. HR - Chorvatsko Klíč. slova hydratation ; wet specimen ; pumping process Vědní obor RIV JA - Elektronika a optoelektronika, elektrotechnika CEP GA102/01/1271 GA ČR - Grantová agentura ČR IBS2065107 GA AV ČR - Akademie věd CEZ AV0Z2065902 - UPT-D Anotace Environmental scanning electron microscope (ESEM) allows the visualisation of specimens that are difficult or impossible to image in a conventional high vacuum SEM. Pressure in the specimen chamber ranges from 1 Pa over 2000 Pa, which permits observation of specimens of being free from charging artefacts and containing different volume of water. But there are some difficulties with the observation of highly wet biological specimens in their natural state because during a pre-pumping process of the specimen chamber an escape of water from the specimen occurs and thus its surface is damaged. The aim of this contribution is to find a method making possible to decrease water escape from the specimen during the initiation pumping process of the ESEM, immediatelly after the inserting of the specimen into the specimen chamber and during an image recording. Pracoviště Ústav přístrojové techniky Kontakt Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Rok sběru 2004
Počet záznamů: 1