Microsatellite region in the murine oncogene src - its primary structure and variability during evolution
1.
SYSNO ASEP
0191701
Druh ASEP
C - Konferenční příspěvek (mezinárodní konf.)
Zařazení RIV
D - Článek ve sborníku
Název
Microsatellite region in the murine oncogene src - its primary structure and variability during evolution
Tvůrce(i)
Fučík, Vladimír (UMG-J) Šindelka, Radek (UMG-J) Beran, Jaroslav (UMG-J) Ferjentsik, Zoltán (UMG-J) Jonák, Jiří (UMG-J)
Zdroj.dok.
Functional Genomics a Disease, Final programme and Abstract book. - Praha : ESF IN FUNCTIONAL GENOMICS, 2003
Rozsah stran
s. 93
Poč.str.
1 s.
Akce
ESF IN FUNCTIONAL GENOMICS: CONFERENCE /1./
Datum konání
14.05.2003-17.05.2003
Místo konání
Praha
Země
CZ - Česká republika
Typ akce
WRD
Jazyk dok.
eng - angličtina
Země vyd.
CZ - Česká republika
Klíč. slova
Mus musculus, c-src introns, microsatellite
Vědní obor RIV
EB - Genetika a molekulární biologie
CEP
GA301/02/0408 GA ČR - Grantová agentura ČR
CEZ
AV0Z5052915 - UMG-J
Anotace
In the fifth intron of the Mus musculus oncogene src a complex microsatellite was discovered that consists of a CA run and 31 bp apart followed by track of TG dinucleotides interrupted by extra Gs in specific intervals. As the 31 bp interlink can assume a hairpin structure, the whole region can be viewed as an imperfect inverted repeat. The microsatellite was sequenced in imbred lines of M. m. musculus and M. m. domesticus, as well as in mice of both subspecies trapped near the hybrid zone. Differences in the length of repeat regions, insertion/deletions and simple base changes characterize the strains. Hybrids between the two species could be identified by sequencing of cloned PCR products. The structure of the microsatellite in more distant species was found to be conspicuously different. Instead of frequent (CA)18-25, we can see only (CA)8 in M. spretus, CATGCGCCCCCCA in Mus pahari and CACCCCCC in Mus caroli. No similar structure is present in the corresponding human src intron.
