Počet záznamů: 1
In vitro distribution and characterization of membrane-associated PLD and PI-PLC in Brassica napus
- 1.
SYSNO ASEP 0172356 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název In vitro distribution and characterization of membrane-associated PLD and PI-PLC in Brassica napus Tvůrce(i) Novotná, Z. (CZ)
Martinec, Jan (UEB-Q) RID, ORCID
Profotová, Bronislava (UEB-Q)
Žďárová, Štěpánka (UEB-Q)
Kader, J. K. (FR)
Valentová, O. (CZ)Zdroj.dok. Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 54, č. 383 (2003), s. 691-698Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Brassica napus ; phospholipases ; plasma membrane Vědní obor RIV CE - Biochemie CEP GA522/00/1332 GA ČR - Grantová agentura ČR LN00A081 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z5038910 - UEB-Q Anotace Two types of phospholipid degrading enzyme, phospholipase D (PLD; EC 3.1.4.4) and phosphatidyl- inositol-specific phospholipase C (PIP2-PLC; PI-PLC 3.1.4.11) were studied during the development of seeds and plants of Brassica napus. PLD exhibits two types of activity; polyphosphoinositide-requiring (PIP2-dependent PLD) and polyphosphoinositide-independent requiring millimolar concentrations of calcium (PLD). Significantly different patterns of activity profiles were found for soluble and membrane-associated forms of all three enzymes within both processes. Membrane-associated PIP2-dependent PLD activity shows the opposite trend when compared to PLD, while the highest PI-PLC activity appears in the same stages of development of seeds and plants as for PLD. In subcellular fractions of hypocotyls of young plants, phospholipases were localized predominantly on plasma membranes. The biochemical characteristics (Ca2+, pH) of all three enzymes associated with plasma membrane vesicles, isolated by partitioning in an aqueous dextran- polyethylene glycol two-phase system, are also described. Direct interaction of PLD with G-proteins under in vitro conditions was not confirmed. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2004
Počet záznamů: 1