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pOp6/LhGR: a stringently regulated and highly responsive dexamethasone-inducible gene expression system for tobacco
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SYSNO ASEP 0023938 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název pOp6/LhGR: a stringently regulated and highly responsive dexamethasone-inducible gene expression system for tobacco Překlad názvu pOp6/LhGR: přísně regulovaný a vysoce responzivní, dexametazonem indukovatelný systém exprese transgenů pro tabák Tvůrce(i) Šámalová, Markéta (BFU-R)
Brzobohatý, Břetislav (BFU-R) RID, ORCID
Moore, Ian (GB)Zdroj.dok. Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 41, - (2005), s. 919-935Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova chemically inducible gene expression ; cytokinin-biosynthetic gene ipt ; tobacco Vědní obor RIV BO - Biofyzika CEP IAA5004001 GA AV ČR - Akademie věd KSK5052113 GA AV ČR - Akademie věd CEZ AV0Z50040507 - BFU-R (2005-2011) Anotace pOp/LhGR, a dexamethasone-inducible derivative of the pOp/LhG4 transcription activation system, and its use in tobacco to regulate expression of uidA (encoding beta-glucuronidase; GUS) and the cytokinin-biosynthetic gene ipt is described. The pOp/LhGR system exhibited stringent regulation and strong induced phenotypes in soil and tissue culture. A single dose of dexamethasone was sufficient to direct cytotoxic levels of ipt expression in soil grown plants although uninduced plants grew normally throughout a complete life cycle. In vitro, induced transcripts were detectable within an hour of dexamethasone application and 1nM dexamethasone was sufficient for half maximal induction of GUS activity. Various methods of dexamethasone application were successfully applied under tissue culture and greenhouse conditions. No inhibitory effects of dexamethasone or LhGR on plant development even with the highest concentrations of inducer were observed. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2006
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