Silencing of carbonic anhydrase I enhances the malignant potential of exosomes secreted by prostatic tumour cells

0509053 - MBÚ 2020 RIV US eng J - Článek v odborném periodiku
Bánová Vulić, R. - Zdurienčíková, M. - Tyčiaková, S. - Benada, Oldřich - Dubrovčáková, M. - Lakota, J. - Škultéty, L'udovít
Silencing of carbonic anhydrase I enhances the malignant potential of exosomes secreted by prostatic tumour cells.
Journal of Cellular and Molecular Medicine. Roč. 23, č. 5 (2019), s. 3641-3655. ISSN 1582-1838. E-ISSN 1582-4934
Grant CEP: GA MŠMT(CZ) LO1509; GA MŠMT(CZ) 8X17052
Institucionální podpora: RVO:61388971
Klíčová slova: carbonic anhydrase I * LC-MS * malignant potential
Obor OECD: Microbiology
Impakt faktor: 4.486, rok: 2019
Způsob publikování: Open access
https://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.14265

We report results showing that the silencing of carbonic anhydrase I (siCA1) in prostatic (PC3) tumour cells has a significant impact on exosome formation. An increased diameter, concentration and diversity of the produced exosomes were noticed as a consequence of this knock-down. The protein composition of the exosomes' cargo was also altered. Liquid chromatography and mass spectrometry analyses identified 42 proteins significantly altered in PC3 siCA1 exosomes compared with controls. The affected proteins are mainly involved in metabolic processes, biogenesis, cell component organization and defense/immunity. Interestingly, almost all of them have been described as 'enhancers' of tumour development through the promotion of cell proliferation, migration and invasion. Thus, our results indicate that the reduced expression of the CA1 protein enhances the malignant potential of PC3 cells.
Trvalý link: http://hdl.handle.net/11104/0299853