Response of the Green Alga Chlamydomonas reinhardtii to the DNA Damaging Agent Zeocin

0508305 - MBÚ 2020 RIV CH eng J - Článek v odborném periodiku
Čížková, Mária - Slavková, Monika - Vítová, Milada - Zachleder, Vilém - Bišová, Kateřina
Response of the Green Alga Chlamydomonas reinhardtii to the DNA Damaging Agent Zeocin.
Cells. Roč. 8, č. 7 (2019), č. článku 735. E-ISSN 2073-4409
Grant CEP: GA MŠMT(CZ) LO1416; GA ČR GA15-09231S
Institucionální podpora: RVO:61388971
Klíčová slova: DNA damage * double-stranded break * zeocin
Obor OECD: Microbiology
Impakt faktor: 4.366, rok: 2019
Způsob publikování: Open access
https://www.mdpi.com/2073-4409/8/7/735

DNA damage is a ubiquitous threat endangering DNA integrity in all living organisms. Responses to DNA damage include, among others, induction of DNA repair and blocking of cell cycle progression in order to prevent transmission of damaged DNA to daughter cells. Here, we tested the effect of the antibiotic zeocin, inducing double stranded DNA breaks, on the cell cycle of synchronized cultures of the green alga Chlamydomonas reinhardtii. After zeocin application, DNA replication partially occurred but nuclear and cellular divisions were completely blocked. Application of zeocin combined with caffeine, known to alleviate DNA checkpoints, decreased cell viability significantly. This was probably caused by a partial overcoming of the cell cycle progression block in such cells, leading to aberrant cell divisions. The cell cycle block was accompanied by high steady state levels of mitotic cyclin-dependent kinase activity. The data indicate that DNA damage response in C. reinhardtii is connected to the cell cycle block, accompanied by increased and stabilized mitotic cyclin-dependent kinase activity.
Trvalý link: http://hdl.handle.net/11104/0299254