The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid

0503711 - ÚŽFG 2020 RIV TR eng J - Článek v odborném periodiku
Pocherniaieva, K. - Güralp, H. - Saito, T. - Pšenička, M. - Tichopád, T. - Janko, Karel - Kašpar, V.
The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid.
Turkish Journal of Fisheries and Aquatic Sciences. Roč. 19, č. 2 (2019), s. 167-174. ISSN 1303-2712. E-ISSN 2149-181X
Grant CEP: GA ČR(CZ) GA17-09807S
Institucionální podpora: RVO:67985904
Klíčová slova: cell cycle * DAPI * synchronization
Obor OECD: Zoology
Impakt faktor: 0.869, rok: 2019
Způsob publikování: Open access
http://trjfas.org/abstract.php?id=1328

Detailed knowledge of early embryo development is a prerequisite for implementation of reproductive biotechnologies. We described the timing of mid-blastula transition in the sturgeon Acipenser ruthenus and hybrid of A. ruthenus and Acipenser gueldenstaedtii. To identify mitotic changes during cell division, the nuclei of embryo blastomeres were sampled every 30 min from 4 to 25 h post-fertilization and stained with 5 mg/ml 4'-6-diaminido-2phenylindole. We observed embryos of hybrids to lose synchrony after the eighth cell division, one cycle earlier than in purebred sterlet embryos. To reveal the timing of zygotic gene activation, developing embryos were dechorionated and injected with 10, 50, 100, 250, or 500 mu g/ml a-Amanitin. Termination of development in embryos was recorded throughout the period from the 1000 cell stage until gastrulation, showing the process of transition from maternal to zygotic gene transcription in sterlet to be of extended duration and not directly linked to mid-blastula transition.
Trvalý link: http://hdl.handle.net/11104/0295511