Study of 2-Nitrofluorene Interaction with DNA at a Glassy Carbon Electrode

0459775 - ÚFCH JH 2017 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Skalová, Štěpánka - Stávková, K. - Vyskočil, V. - Barek, J.
Study of 2-Nitrofluorene Interaction with DNA at a Glassy Carbon Electrode.
XXXVI. Moderní elektrochemické metody. Sborník přednášek. Ústí nad Labem: Best servis, 2016 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), s. 196-200. ISBN 978-80-905221-4-5.
[Moderní elektrochemické metody /36./. Jetřichovice (CZ), 23.05.2016-27.05.2016]
Grant CEP: GA ČR GBP206/12/G151
Institucionální podpora: RVO:61388955
Klíčová slova: DNA biosensor * glassy carbon electrode * voltammetry
Kód oboru RIV: CG - Elektrochemie

2-Nitrofluorene (2-NF) is a nitrated polycyclic aromatic hydrocarbon (NPAH) which occurs as the environmental pollutant. It is a potential carcinogen and mutagen. Interaction of deoxyribonucleic acid (DNA) with 2-NF was monitored using an electrochemical DNA biosensor prepared from a glassy carbon electrode (GCE) and low-molecular-weight DNA by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and square-wave voltammetry (SWV).
There were no damaging interactions observed between DNA and 2-NF using EIS. However, CV shows intercalation of this substance into the DNA structure to form the complex 2-NF–DNA. Intercalation was also observed by SWV, confirming intercalation to reduce the number of electroactive sites and thus reducing the peak heights of adenosine and guanosine.
Trvalý link: http://hdl.handle.net/11104/0259939