Long-term fertilization determines different metabolomic profiles and responses in saplings of three rainforest tree species with different adult canopy position

0476289 - ÚVGZ 2018 RIV US eng J - Článek v odborném periodiku
Gargalo-Garriga, A. - Wright, S. J. - Sardans, J. - Pérez-Trujillo, M. - Oravec, Michal - Večeřová, Kristýna - Urban, Otmar - Fernandez-Martinez, M. - Parella, T. - Peňuelas, J.
Long-term fertilization determines different metabolomic profiles and responses in saplings of three rainforest tree species with different adult canopy position.
PLoS ONE. Roč. 12, č. 5 (2017), č. článku e0177030. ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA MŠMT(CZ) LO1415
Grant ostatní: Akademie věd České Republiky(CZ) M200871201
Výzkumná infrastruktura: CzeCOS II - 90061
Institucionální podpora: RVO:67179843
Klíčová slova: lowland tropical forest * UV-B radiation * terrestrial ecosystem * elemental composition * rainforest
Obor OECD: Environmental sciences (social aspects to be 5.7)
Impakt faktor: 2.766, rok: 2017

Background
Tropical rainforests are frequently limited by soil nutrient availability. However, the response of the metabolic phenotypic plasticity of trees to an increase of soil nutrient availabilities is poorly understood. We expected that increases in the ability of a nutrient that limits some plant processes should be detected by corresponding changes in plant metabolome profile related to such processes.
Methodology/Principal findings
We studied the foliar metabolome of saplings of three abundant tree species in a 15 year field NPK fertilization experiment in a Panamanian rainforest. The largest differences were among species and explained 75% of overall metabolome variation. The saplings of the large canopy species, Tetragastris panamensis, had the lowest concentrations of all identified amino acids and the highest concentrations of most identified secondary compounds. The saplings of the mid canopy species, Alseis blackiana, had the highest concentrations of amino acids coming from the biosynthesis pathways of glycerate-3P, oxaloacetate and alpha-ketoglutarate, and the saplings of the low canopy species, Heisteria concinna, had the highest concentrations of amino acids coming from the pyruvate synthesis pathways.
Conclusions/Significance
The changes in metabolome provided strong evidence that different nutrients limit different species in different ways. With increasing P availability, the two canopy species shifted their metabolome towards larger investment in protection mechanisms, whereas with increasing N availability, the sub-canopy species increased its primary metabolism. The results highlighted the proportional distinct use of different nutrients by different species and the resulting different metabolome profiles in this high diversity community are consistent with the ecological niche theory.
Trvalý link: http://hdl.handle.net/11104/0272820