Arabidopsis non-specific phospholipase C1: Characterization and its involvement in response to heat stress

0451388 - ÚEB 2016 RIV CH eng J - Článek v odborném periodiku
Krčková, Zuzana - Brouzdová, Jitka - Daněk, Michal - Kocourková, Daniela - Rainteau, D. - Ruelland, E. - Valentová, O. - Pejchar, Přemysl - Martinec, Jan
Arabidopsis non-specific phospholipase C1: Characterization and its involvement in response to heat stress.
Frontiers in Plant Science. Roč. 6, NOV 4 (2015), s. 928. ISSN 1664-462X. E-ISSN 1664-462X
Grant CEP: GA ČR(CZ) GAP501/12/1942
Institucionální podpora: RVO:61389030
Klíčová slova: Arabidopsis thaliana * Diacylglycerol * Heat stress
Kód oboru RIV: ED - Fyziologie
Impakt faktor: 4.495, rok: 2015

The Arabidopsis non-specific phospholipase C1 (NPC) protein family is encoded by the genes NPC1 – NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that Arabidopsis NPC1 expressed in Escherichia coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type (WT); npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1 is involved in the plant response to heat.
Trvalý link: http://hdl.handle.net/11104/0252550