In Vivo Determination of Organellar pH Using a Universal Wavelength-Based Confocal Microscopy Approach

0377982 - MBÚ 2013 RIV US eng J - Článek v odborném periodiku
Rodó, A. P. - Váchová, Libuše - Palková, Z.
In Vivo Determination of Organellar pH Using a Universal Wavelength-Based Confocal Microscopy Approach.
PLoS ONE. Roč. 7, č. 3 (2012), s. 1-12. ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA ČR GA204/08/0718
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: GREEN FLUORESCENT PROTEIN * YEAST COLONY DEVELOPMENT * INTRACELLULAR PH
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 3.730, rok: 2012

Many essential cellular processes are affected by transmembrane H+ gradients and intracellular pH (pHi). The research of such metabolic events calls for a non-invasive method to monitor pHi within individual subcellular compartments. We present a novel confocal microscopy approach for the determination of organellar pHi in living cells expressing pHdependent ratiometric fluorescent proteins. Unlike conventional intensity-based fluorometry, our method relies on emission wavelength scans at single-organelle resolution to produce wavelength-based pH estimates both accurate and robust to low-signal artifacts
Trvalý link: http://hdl.handle.net/11104/0209983