Biological autoluminescence as a non-invasive monitoring tool for pulsed electric field effects on yeast cells

0560868 - FZÚ 2023 RIV US eng C - Konferenční příspěvek (zahraniční konf.)
Bereta, M. - Teplan, M. - Chafai, Djamel Eddine - Cifra, Michal
Biological autoluminescence as a non-invasive monitoring tool for pulsed electric field effects on yeast cells.
33rd General Assembly and Scientific Symposium (GASS) of the International-Union-of-Radio-Science (URSI) 2020. New York: IEEE, 2020, č. článku 9232161. ISBN 978-9-4639-6800-3.
[General Assembly and Scientific Symposium (GASS) of the International-Union-of-Radio-Science (URSI) /33./. Řím (IT), 29.08.2020-05.08.2020]
Grant CEP: GA ČR GA18-23597S
Grant ostatní: AV ČR(CZ) SAV-18-11
Program: Bilaterální spolupráce
Institucionální podpora: RVO:68378271 ; RVO:67985882
Klíčová slova: biology * electric fields * monitoring * biomedical measurement * photonics * tools * pulse measure
Obor OECD: Medical laboratory technology (including laboratory samples analysis; Electrical and electronic engineering (URE-Y)
https://ieeexplore.ieee.org/document/9232161/authors#authors

The application of pulsed electric field (PEF) is nowadays becoming a very promising tool for application in medicine or food industry. However, the mechanisms of PEF interaction with living matter are still not fully elucidated. The aim of the presented work is to show the potential application of biological autoluminescence (BAL) for monitoring of pulsed electric field (PEF) biological effects on yeasts. The yeast cell culture (Saccharomyces cerevisiae) is exposed to the pulsed electric field (pulse duration: 0.1-5 ms, pulse number: 1-10, electric field strength: 3 kV.cm(-1)) and the BAL is monitored during exposure. The results indicate detectable differences in BAL dynamics when PEF is applied. Due to its non-invasive and label-free application, the BAL could be used as a beneficial tool for monitoring of PEF biological effects.
Trvalý link: https://hdl.handle.net/11104/0333650