Size and Fluorescence Properties of Algal Photosynthetic Antenna Proteins Estimated by Microscopy

0553484 - MBÚ 2023 RIV CH eng J - Článek v odborném periodiku
Crepin, Aurelia - Belgio, Erica - Šedivá, Barbora - Trsková, Eliška - Cunill-Semanat, Edel - Kaňa, Radek
Size and Fluorescence Properties of Algal Photosynthetic Antenna Proteins Estimated by Microscopy.
International Journal of Molecular Sciences. Roč. 23, č. 2 (2022), č. článku 778. E-ISSN 1422-0067
Grant CEP: GA ČR(CZ) GA19-11494S; GA MŠMT(CZ) LO1416; GA MŠMT(CZ) ED2.1.00/19.0392
Institucionální podpora: RVO:61388971
Klíčová slova: light-harvesting-complex * photosystem-ii * chromera-velia * membrane * supercomplex * acclimation * plants * photosynthesis * antenna proteins * light-harvesting * fluorescence correlation spectroscopy * protein diffusion * microscopy * protein oligomerization * Chromera velia
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 5.6, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/1422-0067/23/2/778

Antenna proteins play a major role in the regulation of light-harvesting in photosynthesis. However, less is known about a possible link between their sizes (oligomerization state) and fluorescence intensity (number of photons emitted). Here, we used a microscopy-based method, Fluorescence Correlation Spectroscopy (FCS), to analyze different antenna proteins at the particle level. The direct comparison indicated that Chromera Light Harvesting (CLH) antenna particles (isolated from Chromera velia) behaved as the monomeric Light Harvesting Complex II (LHCII) (from higher plants), in terms of their radius (based on the diffusion time) and fluorescence yields. FCS data thus indicated a monomeric oligomerization state of algal CLH antenna (at our experimental conditions) that was later confirmed also by biochemical experiments. Additionally, our data provide a proof of concept that the FCS method is well suited to measure proteins sizes (oligomerization state) and fluorescence intensities (photon counts) of antenna proteins per single particle (monomers and oligomers). We proved that antenna monomers (CLH and LHCIIm) are more ´quenched´ than the corresponding trimers. The FCS measurement thus represents a useful experimental approach that allows studying the role of antenna oligomerization in the mechanism of photoprotection.
Trvalý link: http://hdl.handle.net/11104/0331443