Characterization of alba family expression and localization in arabidopsis thaliana generative organs

0545878 - ÚEB 2022 RIV CH eng J - Článek v odborném periodiku
Náprstková, Alena - Malínská, Kateřina - Záveská Drábková, Lenka - Billey, E. - Náprstková, Dagmar - Sýkorová, Eva - Bousquet-Antonelli, C. - Honys, David
Characterization of alba family expression and localization in arabidopsis thaliana generative organs.
International Journal of Molecular Sciences. Roč. 22, č. 4 (2021), č. článku 1652. E-ISSN 1422-0067
Grant CEP: GA ČR(CZ) GA18-07027S; GA MŠMT(CZ) EF16_019/0000738; GA MŠMT(CZ) LM2018129
Institucionální podpora: RVO:61389030 ; RVO:68081707
Klíčová slova: alba * Arabidopsis thaliana * Confocal microscopy * Expression analysis * Flowering * Heat stress * pabp3 * Pollen development * Protein localization
Obor OECD: Plant sciences, botany
Impakt faktor: 6.208, rok: 2021
Způsob publikování: Open access
http://doi.org/10.3390/ijms22041652

ALBA DNA/RNA-binding proteins form an ancient family, which in eukaryotes diversi-fied into two Rpp25-like and Rpp20-like subfamilies. In most studied model organisms, their function remains unclear, but they are usually associated with RNA metabolism, mRNA translatability and stress response. In plants, the enriched number of ALBA family members remains poorly understood. Here, we studied ALBA dynamics during reproductive development in Ara-bidopsis at the levels of gene expression and protein localization, both under standard conditions and following heat stress. In generative tissues, ALBA proteins showed the strongest signal in mature pollen where they localized predominantly in cytoplasmic foci, particularly in regions surrounding the vegetative nucleus and sperm cells. Finally, we demonstrated the involvement of two Rpp25-like subfamily members ALBA4 and ALBA6 in RNA metabolism in mature pollen supported by their co-localization with poly(A)-binding protein 3 (PABP3). Collectively, we demonstrated the engagement of ALBA proteins in male reproductive development and the heat stress response, highlighting the involvement of ALBA4 and ALBA6 in RNA metabolism, storage and/or translational control in pollen upon heat stress. Such dynamic re-localization of ALBA proteins in a controlled, developmentally and environmentally regulated manner, likely reflects not only their redundancy but also their possible functional diversification in plants.
Trvalý link: http://hdl.handle.net/11104/0322504