Počet záznamů: 1  

K+-specific importers Trk1 and Trk2 play different roles in Ca2+ homeostasis and signalling in Saccharomyces cerevisiae cells

  1. 1.
    0542676 - FGÚ 2022 RIV GB eng J - Článek v odborném periodiku
    Zimmermannová, Olga - Felcmanová, Kristina - Sácká, Lenka - Colinet, A. S. - Morsomme, P. - Sychrová, Hana
    K+-specific importers Trk1 and Trk2 play different roles in Ca2+ homeostasis and signalling in Saccharomyces cerevisiae cells.
    FEMS Yeast Research. Roč. 21, č. 3 (2021), č. článku foab015. ISSN 1567-1356. E-ISSN 1567-1364
    Grant CEP: GA ČR(CZ) GA20-04420S
    Institucionální podpora: RVO:67985823
    Klíčová slova: potassium * calcium * K+-transporter * osmotic shock * Trk1 * Trk2 * yeast
    Obor OECD: Microbiology
    Impakt faktor: 2.923, rok: 2021
    Způsob publikování: Omezený přístup
    https://doi.org/10.1093/femsyr/foab015

    The maintenance of K+ and Ca2+ homeostasis is crucial for many cellular functions. Potassium is accumulated in cells at high concentrations, while the cytosolic level of calcium, to ensure its signalling function, is kept at low levels and transiently increases in response to stresses. We examined Ca2+ homeostasis and Ca2+ signalling in Saccharomyces cerevisiae strains lacking plasma-membrane K+ influx (Trk1 and Trk2) or efflux (Tok1, Nha1 and Ena1-5) systems. The lack of K+ exporters slightly increased the cytosolic Ca2+, but did not alter the Ca2+ tolerance or Ca2+-stress response. In contrast, the K+-importers Trk1 and Trk2 play important and distinct roles in the maintenance of Ca2+ homeostasis. The presence of Trk1 was vital mainly for the growth of cells in the presence of high extracellular Ca2+, whilst the lack of Trk2 doubled steady-state intracellular Ca2+ levels. The absence of both K+ importers highly increased the Ca2+ response to osmotic or CaCl2 stresses and altered the balance between Ca2+ flux from external media and intracellular compartments. In addition, we found Trk2 to be important for the tolerance to high KCl and hygromycin B in cells growing on minimal media. All the data describe new interconnections between potassium and calcium homeostasis in S. cerevisiae.
    Trvalý link: http://hdl.handle.net/11104/0320055

     
     
Počet záznamů: 1  

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