G-quadruplexes in H1N1 influenza genomes

0541909 - BFÚ 2022 RIV US eng J - Článek v odborném periodiku
Brázda, Václav - Porubiakova, Otilia - Cantara, Alessio - Bohalova, Natalia - Coufal, Jan - Bartas, Martin - Fojta, Miroslav - Mergny, Jean-Louis
G-quadruplexes in H1N1 influenza genomes.
BMC Genomics. Roč. 22, č. 1 (2021), č. článku 77. ISSN 1471-2164. E-ISSN 1471-2164
Grant CEP: GA MŠMT EF15_003/0000477
Institucionální podpora: RVO:68081707
Klíčová slova: Influenza virus * G-quadruplex * G4Hunter
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 4.558, rok: 2021
Způsob publikování: Open access
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-07377-9

BackgroundInfluenza viruses are dangerous pathogens. Seventy-Seven genomes of recently emerged genotype 4 reassortant Eurasian avian-like H1N1 virus (G4-EA-H1N1) are currently available. We investigated the presence and variation of potential G-quadruplex forming sequences (PQS), which can serve as targets for antiviral treatment.ResultsPQS were identified in all 77 genomes. The total number of PQS in G4-EA-H1N1 genomes was 571. Interestingly, the number of PQS per genome in individual close relative viruses varied from 4 to 12. PQS were not randomly distributed in the 8 segments of the G4-EA-H1N1 genome, the highest frequency of PQS being found in the NP segment (1.39 per 1000nt), which is considered a potential target for antiviral therapy. In contrast, no PQS was found in the NS segment. Analyses of variability pointed the importance of some PQS, even if genome variation of influenza virus is extreme, the PQS with the highest G4Hunter score is the most conserved in all tested genomes. G-quadruplex formation in vitro was experimentally confirmed using spectroscopic methods.ConclusionsThe results presented here hint several G-quadruplex-forming sequences in G4-EA-H1N1 genomes, that could provide good therapeutic targets.
Trvalý link: http://hdl.handle.net/11104/0319404