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Enhancement of the biological autoluminescence by mito-liposomal gold nanoparticle nanocarriers

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    0538002 - ÚFE 2021 RIV CH eng J - Článek v odborném periodiku
    Sardarabadi, H. - Chafai, Djamel Eddine - Gheybi, F. - Sasanpour, P. - Rafii-Tabar, H. - Cifra, Michal
    Enhancement of the biological autoluminescence by mito-liposomal gold nanoparticle nanocarriers.
    Journal of Photochemistry and Photobiology. B - Biology Section. Roč. 204, March (2020), č. článku 111812. ISSN 1011-1344. E-ISSN 1873-2682
    Grant CEP: GA ČR GA18-23597S
    Institucionální podpora: RVO:67985882
    Klíčová slova: Biological autoluminescence signal enhancement * Electron-excited biomolecules * Gold nanoparticles * Mitochondrial-liposomal nanocarrier
    Obor OECD: Electrical and electronic engineering
    Impakt faktor: 6.252, rok: 2020
    Způsob publikování: Omezený přístup
    https://doi.org/10.1016/j.jphotobiol.2020.111812

    One of the most important barriers to the detection of the biological autoluminescence (BAL) from biosystems using a non-invasive monitoring approach, in both the in vivo and the in vitro applications, is its very low signal intensity ( < 1000 photons/s/cm(2)). Experimental studies have revealed that the formation of electron excited species, as a result of reactions of biomolecules with reactive oxygen species (ROS), is the principal biochemical source of the BAL which occurs during the cell metabolism. Mitochondria, as the most important organelles involved in oxidative metabolism, are considered to be the main intracellular BAL source. Hence, in order to achieve the BAL enhancement via affecting the mitochondria, we prepared a novel mitochondrial-liposomal nanocarrier with two attractive features including the intra-liposomal gold nanoparticle synthesizing ability and the mitochondria penetration capability. The results indicate that these nanocarriers (with the average size of 131.1 +/- 20.1 nm) are not only able to synthesize the gold nanoparticles within them (with the average size of 15 nm) and penetrate into the U2OS cell mitochondria, but they are also able to amplify the BAL signals. Our results open new possibilities for the use of biological autoluminescence as a non-invasive and label-free monitoring method in nanomedicine and biotechnology
    Trvalý link: http://hdl.handle.net/11104/0315835

     
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