0535755 - MBÚ 2021 RIV US eng J - Článek v odborném periodiku
Pavlíková, N. - Šrámek, J. - Jelínek, M. - Halada, Petr - Kovář, J.
Markers of acute toxicity of DDT exposure in pancreatic beta-cells determined by a proteomic approach.
PLoS ONE. Roč. 15, č. 10 (2020), č. článku e0229430. ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:61388971
Klíčová slova: persistent organic pollutants * organochlorine pesticides * er stress
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 3.240, rok: 2020
Způsob publikování: Open access
Web výsledku:
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0229430DOI: https://doi.org/10.1371/journal.pone.0229430

Many compounds have the potential to harm pancreatic beta-cells, organochlorine pollutants belong to those compounds. In this work, we aimed to find markers of acute toxicity of p,p'-DDT exposure among proteins expressed in NES2Y human pancreatic beta-cells employing 2-D electrophoresis. We exposed NES2Y cells to a high concentration (150 mu M, LC96 after 72 hours) of p,p'-DDT for 24 and 30 hours and determined proteins with changed expression using 2-D electrophoresis. We have found 22 proteins that changed their expression. They included proteins involved in ER stress (GRP78, and endoplasmin), mitochondrial proteins (GRP75, ECHM, IDH3A, NDUS1, and NDUS3), proteins involved in the maintenance of the cell morphology (EFHD2, TCPA, NDRG1, and ezrin), and some other proteins (HNRPF, HNRH1, K2C8, vimentin, PBDC1, EF2, PCNA, biliverdin reductase, G3BP1, FRIL, and HSP27). The proteins we have identified may serve as indicators of p,p'-DDT toxicity in beta-cells in future studies, including long-term exposure to environmentally relevant concentrations.

Trvalý link: http://hdl.handle.net/11104/0313698