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Combined effect of lasioglossin LL-III derivative with azoles against Candida albicans virulence factors: biofilm formation, phospholipases, proteases and hemolytic activity

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    0524988 - ÚOCHB 2021 RIV GB eng J - Článek v odborném periodiku
    Vaňková, Eva - Kašparová, Petra - Dulíčková, Nikola - Čeřovský, Václav
    Combined effect of lasioglossin LL-III derivative with azoles against Candida albicans virulence factors: biofilm formation, phospholipases, proteases and hemolytic activity.
    FEMS Yeast Research. Roč. 20, č. 3 (2020), č. článku foaa020. ISSN 1567-1356. E-ISSN 1567-1364
    Grant CEP: GA MZd(CZ) NV16-27726A
    Institucionální podpora: RVO:61388963
    Klíčová slova: antimicrobial peptides * azoles * biofilm formation * Candida albicans * LL-III derivative * virulence factors
    Obor OECD: Microbiology
    Impakt faktor: 2.796, rok: 2020
    Způsob publikování: Omezený přístup
    https://academic.oup.com/femsyr/article-abstract/20/3/foaa020/5824167?redirectedFrom=fulltext

    Candida albicans has several virulence factors at its disposal, including yeast–hyphal transition associated with biofilm formation, phospholipases, proteases and hemolytic activity, all of which contribute to its pathogenesis. We used synthetic derivative LL-III/43 of antimicrobial peptide lasioglossin LL-III to enhance effect of azoles on attenuation of C. albicans virulence factors. LL-III/43 was able to inhibit initial adhesion or biofilm formation of C. albicans strains at 50 µM. Azoles, however, were ineffective at this concentration. Using fluorescently labeled LL-III/43, we observed that peptide covered C. albicans cells, partially penetrated through their membranes and then accumulated inside cells. LL-III/43 (25 µM) in combination with clotrimazole prevented biofilm formation already at 3.1 µM clotrimazole. Neither LL-III/43 nor azoles were able to significantly inhibit phospholipases, proteases, or hemolytic activity of C. albicans. LL-III/43 (25 µM) and clotrimazole (50 µM) in combination decreased production of these virulence factors, and it completely attenuated its hemolytic activity. Scanning electron microscopy showed that LL-III/43 (50 µM) prevented C. albicans biofilm formation on Ti-6Al-4 V alloy used in orthopedic surgeries and combination of LL-III/43 (25 µM) with clotrimazole (3.1 µM) prevented biofilm formation on urinary catheters. Therefore, mixture of LL-III/43 and clotrimazole is suitable candidate for future pharmaceutical research.
    Trvalý link: http://hdl.handle.net/11104/0309197

     
     
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