Comparison of oligosaccharide labeling employing various derivatization chemistries

0509074 - ÚIACH 2020 PL eng A - Abstrakt
Křenková, Jana - Čmelík, Richard - Přikryl, Jan
Comparison of oligosaccharide labeling employing various derivatization chemistries.
CECE 2019. Gdańsk: University of Gdańsk, 2019. s. 14-14.
[CECE 2019. International Interdisciplinary Meeting on Bioanalysis /16./. 24.09.2019-26.09.2019, Gdańsk]
Grant CEP: GA ČR(CZ) GA18-00062S
Grant ostatní: AV ČR(CZ) MTA-19-06
Program: Bilaterální spolupráce
Institucionální podpora: RVO:68081715
Klíčová slova: oligosaccharide/glycan analysis * oligosaccharide labeling * liquid chromatography * capillary electrophoresis
Obor OECD: Analytical chemistry

In this work, we compare oligosaccharide labeling by two negatively charged fluorescent labels, APTS and Cascade Blue hydrazide. Effectiveness of the labeling chemistries were investigated by maltopentaose and 4-hydroxybenzaldehyde followed by LC/UV and LC-MS analysis. Finally, the more effective hydrazone formation technique was applied for oligosaccharide and N-linked glycan analysis by CE/LIF.
Trvalý link: http://hdl.handle.net/11104/0299868