Formaldehyde fixation is detrimental to actin cables in glucose-depleted S. cerevisiae cells

0462340 - MBÚ 2017 RIV AT eng J - Článek v odborném periodiku
Vašicová, Pavla - Rinnerthaler, M. - Hašková, Danuša - Nováková, Lenka - Malcová, Ivana - Breitenbach, M. - Hašek, Jiří
Formaldehyde fixation is detrimental to actin cables in glucose-depleted S. cerevisiae cells.
Microbial cell. Roč. 3, č. 5 (2016), s. 206-214. E-ISSN 2311-2638
Grant CEP: GA ČR GAP305/12/0480; GA ČR(CZ) GA16-05497S; GA MŠMT(CZ) 7AMB16AT006
Institucionální podpora: RVO:61388971
Klíčová slova: Abp140-GFP * Abp1-RFP * Actin cables
Kód oboru RIV: EE - Mikrobiologie, virologie

Actin filaments form cortical patches and emanating cables in fermenting cells of Saccharomyces cerevisiae. This pattern has been shown to be depolarized in glucose-depleted cells after formaldehyde fixation and staining with rhodamine-tagged phalloidin. Loss of actin cables in mother cells was remarkable. Here we extend our knowledge on actin in live glucosedepleted cells co-expressing the marker of actin patches (Abp1-RFP) with the marker of actin cables (Abp140-GFP). Glucose depletion resulted in appearance of actin patches also in mother cells. However, even after 80 min of glucose deprivation these cells showed a clear network of actin cables labeled with Abp140-GFP in contrast to previously published data.
Trvalý link: http://hdl.handle.net/11104/0261826