Počet záznamů: 1
Second harmonic imaging of chloroplasts using the two-photon laser scanning microscope
- 1.0343182 - ÚVGZ 2011 RIV GB eng J - Článek v odborném periodiku
Reshak, Ali H - Sarafis, V. - Heintzmann, R.
Second harmonic imaging of chloroplasts using the two-photon laser scanning microscope.
Micron. Roč. 40, č. 3 (2009), s. 378-385. ISSN 0968-4328. E-ISSN 1878-4291
Výzkumný záměr: CEZ:AV0Z60870520
Klíčová slova: 2PLSM * collagen * generatin * scattering * membrane * fibrils
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 1.626, rok: 2009
Background: Second harmonic information reveals information about the structure of spatially oriented structures with an asymmetry. We study the second harmonic images of the grana and intergranal parts of chloroplasts in the leaves of the shade moss Plagiomnium affine. Results: A two-photon microscope with blocking filters to suppress fluorescence generated both forward and backward second harmonic illumination to be captured. In the starch free chloroplasts of the single cell layered laminae strong second harmonic generation (SHG) from the granal regions was emitted. Upon illumination the chloroplasts changed their orientation affecting the SHG signal. Chloroplast is reoriented upon illumination.
Trvalý link: http://hdl.handle.net/11104/0185716
Počet záznamů: 1