Počet záznamů: 1
Synthesis of nucleoside and nucleotide conjugates of bile acids, and polymerase construction of bile acid-functionalized DNA
- 1.0342359 - ÚOCHB 2011 RIV GB eng J - Článek v odborném periodiku
Ikonen, Satu - Macíčková-Cahová, Hana - Pohl, Radek - Šanda, Miloslav - Hocek, Michal
Synthesis of nucleoside and nucleotide conjugates of bile acids, and polymerase construction of bile acid-functionalized DNA.
Organic & Biomolecular Chemistry. Roč. 8, č. 5 (2010), s. 1194-1201. ISSN 1477-0520. E-ISSN 1477-0539
Grant CEP: GA MŠMT LC512; GA ČR GA203/09/0317
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: steroids * nucleosides * nucleoside triphosphates * DNA polymerase
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 3.451, rok: 2010
Sonogashira cross-coupling reactions of 5-iodopyrimidine or 7-iodo-7-deazaadenine nucleosides with bile acid-derived terminal acetylenes gave the corresponding bile acid-nucleoside conjugates. Analogous reactions of halogenated nucleoside triphosphates gave directly bile acid-modified dNTPs. Enzymatic incorporation of these modified nucleotides to DNA was successfully performed using Phusion polymerase for primer extension.
Trvalý link: http://hdl.handle.net/11104/0185111
Počet záznamů: 1