Molecular cloning, E.coli expression and purification of SCFV antibody fragments of diagnostic/therapeutic interest

0191540 - UMG-J 20033034 RIV CZ eng K - Konferenční příspěvek (tuzemská konf.)
Král, Vlastimil - Fábry, Milan - Hořejší, Magdalena - Závada, Jan - Sedláček, Juraj
Molecular cloning, E.coli expression and purification of SCFV antibody fragments of diagnostic/therapeutic interest.
Material Structure in Chemistry, Biology, Physics and Technology. Praha,: Czech and Slovak Crystallographic Association, 2003 - (Kužel, R.; Hašek, J.; Fiala, J.; Weiss, Z.), s. 56. ISSN 1211-5894.
[Meeting of the Czech and Slovak Structural Biologists /2./. Nové Hrady (CZ), 13.03.2003-15.03.2003]
Grant CEP: GA ČR GV203/98/K023
Výzkumný záměr: CEZ:AV0Z5052915
Klíčová slova: cloning * purification * scFv
Kód oboru RIV: CE - Biochemie

We describe molecular cloning, expression, purification and properties of two single chain antibody variable fragments (scFv) of potential diagnostic use, namely scFv M75 and scFv Tu-20. The former scFv is derived from a monoclonal antibody M75 specific for a cell surface protein MN/CA IX, strongly associated with many types of human carcinomas. The latter scFv is derived from a monoclonal antibody TU-20 specific for neuronal beta-III-tubulin.
Trvalý link: http://hdl.handle.net/11104/0087284