Natural plant hormones cytokinins increase stress resistance and longevity of Caenorhabditis elegans

0488707 - ÚEB 2019 RIV US eng J - Článek v odborném periodiku
Kadlecová, Alena - Jirsa, Tomáš - Novák, Ondřej - Kammenga, J. - Strnad, Miroslav - Voller, Jiří
Natural plant hormones cytokinins increase stress resistance and longevity of Caenorhabditis elegans.
Biogerontology. Roč. 19, č. 2 (2018), s. 109-120. ISSN 1389-5729. E-ISSN 1573-6768
Grant CEP: GA MŠMT(CZ) LO1204; GA MŠMT(CZ) LO1304
Institucionální podpora: RVO:61389030
Klíčová slova: Aging * Caenorhabditis elegans * Cytokinin * Kinetin * Phytohormones * Topolin * Zeatin
Obor OECD: Plant sciences, botany
Impakt faktor: 3.805, rok: 2018

Cytokinins are phytohormones that are involved in many processes in plants, including growth, differentiation and leaf senescence. However, they also have various activities in animals. For example, kinetin and trans-zeatin can reduce levels of several aging markers in human fibroblasts. Kinetin can also protect mice against oxidative and glyoxidative stress, and prolong fruit flies’ lifespan. Additionally, several cytokinins are currently used in cosmetics. To extend knowledge of the breadth of cytokinins’ activities, we examined effects of natural cytokinin bases on the model nematode Caenorhabditis elegans. We found that kinetin, para-topolin and meta-topolin prolonged the lifespan of C. elegans. Kinetin also protected the organism against oxidative and heat stress. Furthermore, our results suggest that presence of reactive oxygen species, but not DAF-16 (the main effector of the insulin/insulin-like growth factor signaling pathway), is required for the beneficial effects of kinetin. Ultra-high performance liquid chromatography-tandem mass spectrometric analysis showed that kinetin is unlikely to occur naturally in C. elegans, but the worm efficiently absorbs and metabolizes it into kinetin riboside and kinetin riboside-5′-monophosphate.
Trvalý link: http://hdl.handle.net/11104/0283257