A MODIFIED MATHEMATICAL MODEL OF HUMAN VENTRICULAR CARDIOMYOCYTE INCORPORATING SEPARATE T-TUBULAR AND SURFACE DYADS AND SUBMEMBRANE SPACES

0483584 - ÚT 2018 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Pásek, Michal - Bébarová, M. - Christé, G.
A MODIFIED MATHEMATICAL MODEL OF HUMAN VENTRICULAR CARDIOMYOCYTE INCORPORATING SEPARATE T-TUBULAR AND SURFACE DYADS AND SUBMEMBRANE SPACES.
Engineering Mechanics 2017. Brno: University of technology, Institute of Solid Mechanics, Mechatronics and Biomechanics, 2017 - (Fuis, V.), s. 750-753. ISBN 978-80-214-5497-2. ISSN 1805-8248.
[Engineering Mechanics 2017. Svratka (CZ), 15.05.2017-18.05.2017]
Institucionální podpora: RVO:61388998
Klíčová slova: human ventricular cell model * t-tubules * dyads * subsarcolemmal spaces * calcium cycling
Obor OECD: Biophysics

Intracellular Ca2+ load and Ca2+ transient are considerably dependent on distribution of sarcolemmal Ca2+ pump and Na+-Ca2+ exchanger between the t-tubular and surface membranes in the presence of separate dyadic and subsarcolemmal spaces in rat ventricular cell model. To explore analogical phenomenon in human, we modified our previously published model of human ventricular myocyte. When the t-tubular fractions of Na+-Ca2+ exchanger and of sarcolemmal Ca2+ pump were increased to the newly proposed value of 0.95 in the modified model, the following changes were observed at 1 Hz steady-state stimulation: a shortening of the action potential duration at 90% repolarisation by 6% and an increase of the cytosolic Ca2+ transient by 22%. Further analysis revealed a critical role of Ca2+ concentration changes in the subsarcolemmal spaces and consequent change in cellular Ca2+ cycling in this effect.
Trvalý link: http://hdl.handle.net/11104/0279791