Počet záznamů: 1
Additions of Thiols to 7-Vinyl-7-deazaadenine Nucleosides and Nucleotides. Synthesis of Hydrophobic Derivatives of 2'-Deoxyadenosine, dATP and DNA
- 1.0470348 - ÚOCHB 2017 RIV US eng J - Článek v odborném periodiku
Slavíčková, Michaela - Pohl, Radek - Hocek, Michal
Additions of Thiols to 7-Vinyl-7-deazaadenine Nucleosides and Nucleotides. Synthesis of Hydrophobic Derivatives of 2'-Deoxyadenosine, dATP and DNA.
Journal of Organic Chemistry. Roč. 81, č. 22 (2016), s. 11115-11125. ISSN 0022-3263. E-ISSN 1520-6904
Grant CEP: GA ČR GA14-04289S
Institucionální podpora: RVO:61388963
Klíčová slova: terminal deoxynucleotidyl transferase * enzymatic synthesis * cross linking
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 4.849, rok: 2016
http://pubs.acs.org/doi/abs/10.1021/acs.joc.6b02098
Additions of alkyl-or arylthiols to 7-vinyl-7-deaza2'-deoxyadenosine gave a series of 7-[2-(alkyl-or arylsulfanyl)ethyl]-7-deaza-2'-deoxyadenosines in 45-85% yields. The nucleosides were converted to 5'-0-mono-(dA(SR)MP) or triphosphates (dA(SR)TP) by phosphorylation. The modified triphosphates were also prepared by thiol addition to 7-vinyl-7deaza-dATP. The triphosphates dASRTP were good substrates for DNA polymerases useful in the enzymatic synthesis of base-modified oligonucleotides (ONs) or DNA containing flexibly linked hydrophobic substituents in the major groove. Primer extension was used for the synthesis of ONs with one or several modifications, PCR was used for the synthesis of heavily modified DNA, whereas terminal deoxynucleotidyl transferase was used for a single-nucleotide labeling of the 3'-end.
Trvalý link: http://hdl.handle.net/11104/0268048
Název souboru Staženo Velikost Komentář Verze Přístup 0470348.pdf 10 3.1 MB Vydavatelský postprint povolen
Počet záznamů: 1