Sequencing of 15622 gene-bearing BACs clarifies the gene-dense regions of the barley genome

0451006 - ÚEB 2016 RIV GB eng J - Článek v odborném periodiku
Munoz-Amatriain, M. - Lonardi, S. - Luo, M.C. - Madishetty, K. - Svensson, J.T. - Moscou, M. J. - Wanamaker, S. - Kudrna, D. - Zheng, J. - Šimková, Hana - Doležel, Jaroslav - Grimwood, J. - Mammadov, J. - Close, T.J. … celkem 49 autorů
Sequencing of 15622 gene-bearing BACs clarifies the gene-dense regions of the barley genome.
Plant Journal. Roč. 84, č. 1 (2015), s. 216-227. ISSN 0960-7412. E-ISSN 1365-313X
Grant CEP: GA MŠMT(CZ) LO1204
Institucionální podpora: RVO:61389030
Klíčová slova: Barley * Hordeum vulgare L * BAC sequencing
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 5.468, rok: 2015

Barley (Hordeum vulgare L.) possesses a large and highly repetitive genome of 5.1Gb that has hindered the development of a complete sequence. In 2012, the International Barley Sequencing Consortium released a resource integrating whole-genome shotgun sequences with a physical and genetic framework. However, because only 6278 bacterial artificial chromosome (BACs) in the physical map were sequenced, fine structure was limited. To gain access to the gene-containing portion of the barley genome at high resolution, we identified and sequenced 15622 BACs representing the minimal tiling path of 72052 physical-mapped gene-bearing BACs. This generated 1.7Gb of genomic sequence containing an estimated 2/3 of all Morex barley genes. Exploration of these sequenced BACs revealed that although distal ends of chromosomes contain most of the gene-enriched BACs and are characterized by high recombination rates, there are also gene-dense regions with suppressed recombination. We made use of published map-anchored sequence data from Aegilops tauschii to develop a synteny viewer between barley and the ancestor of the wheat D-genome. Except for some notable inversions, there is a high level of collinearity between the two species. The software HarvEST:Barley provides facile access to BAC sequences and their annotations, along with the barley-Ae.tauschii synteny viewer. These BAC sequences constitute a resource to improve the efficiency of marker development, map-based cloning, and comparative genomics in barley and related crops. Additional knowledge about regions of the barley genome that are gene-dense but low recombination is particularly relevant.
Trvalý link: http://hdl.handle.net/11104/0252160