Construction and Application of Flow Enzymatic Biosensor Based of Silver Solid Amalgam Electrode for Determination of Sarcosine

0446079 - ÚFCH JH 2016 RIV DE eng J - Článek v odborném periodiku
Josypčuk, Oksana - Barek, J. - Josypčuk, Bohdan
Construction and Application of Flow Enzymatic Biosensor Based of Silver Solid Amalgam Electrode for Determination of Sarcosine.
Electroanalysis. Roč. 27, č. 11 (2015), s. 2559-2566. ISSN 1040-0397. E-ISSN 1521-4109
Grant CEP: GA ČR GBP206/12/G151; GA ČR GAP206/11/1638
Institucionální podpora: RVO:61388955
Klíčová slova: biosensors * sarcosine * silver solid amalgam electrode
Kód oboru RIV: CG - Elektrochemie
Impakt faktor: 2.471, rok: 2015

In this paper, the flow amperometric enzymatic biosensor based on polished silver solid amalgam electrode for determination of sarcosine in model sample under flow injection analysis conditions is presented. The biosensor works on principle of electrochemical detection of oxygen decrease during enzymatic reaction which is directly proportional to the concentration of sarcosine in sample. The whole preparation process takes about 3 h. The RSD of repeatability of 10 consecutive measurements is 1.6% (csarcosine=1.0x10-4 moldm-3). Under optimal conditions the calibration dependence was linear in the range 7.5x10-6 – 5.0x10-4 moldm-3 and limit of detection was 2.0x10-6 moldm-3.
Trvalý link: http://hdl.handle.net/11104/0248062