The long-term effect of zinc soil contamination on selected free amino acids playing an important role in plant adaptation to stress and senescence

0429992 - ÚEB 2015 RIV US eng J - Článek v odborném periodiku
Pavlíková, D. - Zemanová, V. - Procházková, Dagmar - Pavlík, Milan - Száková, J. - Wilhelmová, Naděžda
The long-term effect of zinc soil contamination on selected free amino acids playing an important role in plant adaptation to stress and senescence.
Ecotoxicology and Enviromental Safety. Roč. 100, FEB 2014 (2014), s. 166-170. ISSN 0147-6513. E-ISSN 1090-2414
Grant CEP: GA ČR(CZ) GAP501/11/1239
Institucionální podpora: RVO:61389030
Klíčová slova: Amino acid * Cytokinins * Senescence
Kód oboru RIV: EF - Botanika
Impakt faktor: 2.762, rok: 2014

Increased endogenous plant cytokinin (CK) content through transformation with an isopentyl transferase (ipt) gene has been associated with improved plant stress tolerance. The objective of this study is to determine amino acid changes associated with elevated CK production in ipt transgenic tobacco (Nicotiana tabacum L, cv. Wisconsin 38). Nontransformed (WT) and transformed tobacco plants with ipt gene controlled by senescence-activated promoter (SAG) were exposed to zinc soil contamination (tested levels Zn1=250, Zn2=500, Zn3=750 mg kg(-1) soil). The Zn effect on plant stress metabolism resulted in changes in levels of selected free amino acids playing an important role in adaptation to stress and plant senescence (alanine, leucine, proline, methionine and gamma-aminobutyrate) and differed for transformed and nontransformed tobacco plants. Analyses of amino adds confirmed that SAG tobacco plants had improved zinc tolerance compared with the WT plants. The enhanced Zn tolerance of SAG plants was associated with the maintenance of accumulation of proline, methionine and gamma-aminobutyrate. The concentrations of leucine and alanine did not show significant differences between plant lines.
Trvalý link: http://hdl.handle.net/11104/0234936