Cell Wall Components of Leptosphaeria maculans Enhance Resistance of Brassica napus

0397255 - ÚEB 2014 RIV US eng J - Článek v odborném periodiku
Kim, P.D. - Šašek, Vladimír - Burketová, Lenka - Čopíková, J. - Synytsya, A. - Jindřichová, Barbora - Valentová, O.
Cell Wall Components of Leptosphaeria maculans Enhance Resistance of Brassica napus.
Journal of Agricultural and Food Chemistry. Roč. 61, č. 22 (2013), s. 5207-5214. ISSN 0021-8561. E-ISSN 1520-5118
Grant CEP: GA ČR GA522/08/1581; GA MZe QH81201
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: elicitor * oligosaccharide * Leptosphaeria maculans
Kód oboru RIV: GF - Choroby, škůdci, plevely a ochrana rostlin
Impakt faktor: 3.107, rok: 2013

Preparations with elicitation activity were obtained from the mycelium of Leptosphaeria maculans, a fungal pathogen of oilseed rape (Brassica napus). Crude delipidated and deproteinized extract from fungal cell walls induced expression of pathogenesis related gene 1 (PR1), hydrogen peroxide accumulation, and enhanced resistance of B. napus plants toward infection by L. maculans. Elicitation activity significantly decreased after treatment of a crude extract with alpha- or beta-glucanase. Monosaccharide composition analysis of a crude extract purified by ion-exchange chromatography revealed glucose (similar to 58 mol %), mannose (similar to 22 mol %), and galactose (similar to 18 mol %) as the major sugars. FT-IR and NMR spectra confirmed the presence of both carbohydrate and polypeptide components in the purified product. Correlation NMR experiments defined trisaccharide bound to O-3 of serine residue alpha-D-Glcp-(1 -> 2)-beta-D-Galf-(1 -> 6)-alpha-D-Manp-(1 -> 3)-L-Ser. Terminal alpha-D-Glcp and (1 -> 6)-beta-D-glucan were also detected. The obtained results strongly support the conclusion that these carbohydrated induce defence response in B. napus plants.
Trvalý link: http://hdl.handle.net/11104/0224900