Physical Mapping Integrated with Syntenic Analysis to Characterize the Gene Space of the Long Arm of Wheat Chromosome 1A

0396782 - ÚEB 2014 RIV US eng J - Článek v odborném periodiku
Lucas, S. - Akpinar, B.A. - Kantar, M. - Weinstein, Z. - Šafář, Jan - Šimková, Hana - Frenkel, Z. - Berges, H. - Doležel, Jaroslav - Budak, H. … celkem 16 autorů
Physical Mapping Integrated with Syntenic Analysis to Characterize the Gene Space of the Long Arm of Wheat Chromosome 1A.
PLoS ONE. Roč. 8, č. 4 (2013). ISSN 1932-6203. E-ISSN 1932-6203
Grant ostatní: GA MŠk(CZ) ED0007/01/01
Program: ED
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: BREAD WHEAT * COMPLEX GENOMES * MAP
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 3.534, rok: 2013

Background: Bread wheat (Triticum aestivum L.) is one of the most important crops worldwide and its production faces pressing challenges, the solution of which demands genome information. However, the large, highly repetitive hexaploid wheat genome has been considered intractable to standard sequencing approaches. Therefore the International Wheat Genome Sequencing Consortium (IWGSC) proposes to map and sequence the genome on a chromosome-by-chromosome basis. Methodology/Principal Findings: We have constructed a physical map of the long arm of bread wheat chromosome 1A using chromosome-specific BAC libraries by High Information Content Fingerprinting (HICF). Two alternative methods (FPC and LTC) were used to assemble the fingerprints into a high-resolution physical map of the chromosome arm. A total of 365 molecular markers were added to the map, in addition to 1122 putative unique transcripts that were identified by microarray hybridization. The final map consists of 1180 FPC-based or 583 LTC-based contigs. Conclusions/Significance: The physical map presented here marks an important step forward in mapping of hexaploid bread wheat. The map is orders of magnitude more detailed than previously available maps of this chromosome, and the assignment of over a thousand putative expressed gene sequences to specific map locations will greatly assist future functional studies. This map will be an essential tool for future sequencing of and positional cloning within chromosome 1A.
Trvalý link: http://hdl.handle.net/11104/0224500