Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato

0395286 - BC 2014 RIV US eng J - Článek v odborném periodiku
Kodrík, Dalibor - Kludkiewicz, Barbara - Navrátil, Oldřich - Skoková Habuštová, Oxana - Horáčková, V. - Svobodová, Z. - Vinokurov, Konstantin - Sehnal, František
Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato.
Applied Biochemistry and Biotechnology. Roč. 171, č. 1 (2013), s. 209-224. ISSN 0273-2289. E-ISSN 1559-0291
Grant CEP: GA MZe QI91A229
Institucionální podpora: RVO:60077344 ; RVO:61389030
Klíčová slova: genetically modified organism * silk protease inhibitor * protease
Kód oboru RIV: ED - Fyziologie
Impakt faktor: 1.687, rok: 2013
http://link.springer.com/content/pdf/10.1007%2Fs12010-013-0325-9.pdf

Several recombinant derivatives of silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subtilisin and proteinase K. A construct of the gene was introduced into Agrobacterium tumefaciens that was then used for genetic transformation of the potato variety Velox. In vitro tests showed increased resistance to the late blight Phytophthora infestans in several transformed lines. No effect was seen on biological characteristics of Spodoptera littoralis indicating that the transformed potatoes may be harmless to non-target organisms.
Trvalý link: http://hdl.handle.net/11104/0225670