0617300 - ÚMG 2025 RIV US eng J - Článek v odborném periodiku
Špoutil, František - Dlugošová, Sylvie - Varga, Igor - Aranaz-Novaliches, Gorreti - Novosadová, Vendula - Procházková, Michaela - Sedláček, Radislav - Procházka, Jan
Semi-Automated MicroCT Analysis of Bone Anatomy and Mineralization in Mouse Models.
Current Protocols. Roč. 4, č. 2 (2024), č. článku e980. ISSN 2691-1299. E-ISSN 2691-1299
Grant CEP: GA MŠMT LM2023036; GA MŠMT EF18_046/0015861; GA MŠMT EF16_013/0001789; GA ČR GJ19-19025Y
Institucionální podpora: RVO:68378050
Klíčová slova: cortical bone * femur * lumbar vertebra * microCT * mineralization * skeleton phenotyping * trabecular bone
Obor OECD: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Způsob publikování: Omezený přístup
Web výsledku:
https://currentprotocols.onlinelibrary.wiley.com/doi/epdf/10.1002/cpz1.980

DOI: https://doi.org/10.1002/cpz1.980

The skeletal system mirrors several processes in the vertebrate body that impact developmental malfunctions, hormonal disbalance, malfunction of calcium metabolism and turn over, and inflammation processes such as arthrosis. X-ray micro computed tomography is a useful tool for 3D in situ evaluation of the skeletal system in a time-related manner, but results depend highly on resolution. Here, we provide the methodological background for a graduated evaluation from whole-body analysis of skeletal morphology and mineralization to high-resolution analysis of femoral and vertebral microstructure. We combine an expert-based evaluation with a machine-learning-based computational approach, including pre-setup analytical task lists. © 2024 Wiley Periodicals LLC. Basic Protocol 1: In vivo microCT scanning and skeletal analysis in mice. Basic Protocol 2: Ex vivo high-resolution microCT scanning and microstructural analysis of the femur and L4 vertebra.
Trvalý link: https://hdl.handle.net/11104/0364422