0605301 - ÚMG 2026 RIV NL eng J - Článek v odborném periodiku
Miladinović, Ana - Antiga, Ludovica - Venit, Tomáš - Bayona-Hernandez, A. - Červenka, Jakub - Labala, Rajendra Kumar - Kolář, Michal - Castano, E. - Sztacho, Martin - Hozák, Pavel
The perinucleolar compartment and the oncogenic super-enhancers are part of the same phase-separated structure filled with phosphatidylinositol 4,5bisphosphate and long noncoding RNA HANR.
Advances in Biological Regulation. Roč. 95, Jan (2025), č. článku 101069. ISSN 2212-4926. E-ISSN 2212-4926
Grant CEP: GA MŠMT(CZ) LM2023050; GA ČR(CZ) GA17-09103S; GA MŠMT(CZ) LM2023055; GA MŠMT LX22NPO5102
GRANT EU: European Commission(XE) CA19105 - EpiLipidNET
Výzkumná infrastruktura: Czech-BioImaging III - 90250
Institucionální podpora: RVO:68378050 ; RVO:67985904
Klíčová slova: Phase separation * Perinucleolar compartment * PNC * Oncogenic super-enhancers * Long non-coding RNA * Phosphatidylinositol 4,5-bisphosphate * PIP2 * HANR
Obor OECD: Oncology
Způsob publikování: Open access
Web výsledku:
https://www.sciencedirect.com/science/article/pii/S2212492624000575?via%3DihubDOI: https://doi.org/10.1016/j.jbior.2024.101069

The liquid-liquid phase separation in the cell nucleus regulates various processes such as gene regulation and transcription control, chromatin organization, and DNA repair. A plethora of proteins and RNAs contribute to the formation of biomolecular condensates and recently, several nuclear phosphoinositides were shown to be a part of these membrane-less complexes within the nucleus as well. Here we lipid-interacting RNA sequencing (LIPRNAseq) and confocal microscopy to uncover the RNA-binding capacity and localization of phosphatidylinositol 4,5 bisphosphate (PIP2). We discovered the consensus PIP2-binding AU-rich RNA motif and identified long non-coding RNA HANR (lncHANR) to colocalize with PIP2 in the proximity to the nucleolus in the perinucleolar compartment (PNC). Colocalization studies with different nuclear markers reveal that PIP2-HANR presence in the PNC correlates with oncogenic super-enhancers, and both PNC and oncogenic enhancers are part of the same structure. As lncHANR, PNC, and oncogenic super-enhancers are associated with cancer cell lines and tumors, we suggest that they can serve as interchangeable prognostic markers. Understanding of the interplay between lipid metabolism, and lncRNAs in subnuclear compartment phase separation can lead to future improvement in treatment strategies and personalized cancer management approaches.
Trvalý link: https://hdl.handle.net/11104/0362955