0605183 - MBÚ 2026 RIV NL eng J - Článek v odborném periodiku
Sushytskyi, Leonid - Synytsya, A. - Lukáč, Pavol - Rajsiglová, Lenka - Capek, P. - Pohl, Radek - Bleha, R. - Vannucci, Luca - Smrž, Daniel - Čopíková, J. - Kaštánek, P.
Immunologically active cell wall polysaccharides of green microalga Dictyosphaerium chlorelloides (Chlorellacea).
Carbohydrate Polymers. Roč. 353, April 1 (2025), č. článku 123242. ISSN 0144-8617. E-ISSN 1879-1344
Grant CEP: GA MŠMT LX22NPO5102; GA MPO FV10155
Institucionální podpora: RVO:61388971 ; RVO:61388963
Klíčová slova: Dictyosphaerium chlorelloides * Immunomodulating activity * Microalgae biomass * Structure * Water-soluble polysaccharides
Obor OECD: Microbiology; Analytical chemistry (UOCHB-X)
Impakt faktor: 10.7, rok: 2023 ; AIS: 1.34, rok: 2023
Způsob publikování: Open access
Web výsledku:
https://www.sciencedirect.com/science/article/pii/S0144861725000232?via%3DihubDOI: https://doi.org/10.1016/j.carbpol.2025.123242

Dictyosphaerium chlorelloides is a green microalga from the Chlorella clade that produces highly viscous exocellular polysaccharides. The cell wall polysaccharides of this alga have not been studied in detail. In this article, water-soluble polysaccharides from D. chlorelloides biomass were extracted with hot water and purified by preparative chromatography. The composition, structural features and molecular masses of subsequently eluted fractions F1, F2, F3, F4 and F5 (minor) were determined. Three high-yield products F1, F3 and F4 consisted mainly of galactopyranosyl, 2-O-methyl-galactopyranosyl, rhamnopyranosyl and mannopyranosyl units at different proportions, while F2 was rich in glucose. Immunoactivity of these fractions was evidenced in a mixed population of immune cells derived from mice spleens after incubation with polysaccharides by flow cytometry, MTT and Immunospot assays. These fractions, except F2, demonstrated selective immunostimulant activity, and the F1 fraction induced the most potent effect, closely followed by the F3 and F4 fractions. The in vivo mechanism of their action is associated with the activation of innate immunity and shapes the immune response to the Th1 type.
Trvalý link: https://hdl.handle.net/11104/0365355
Vědecká data: data součástí článku