0601447 - MBÚ 2026 RIV NL eng J - Journal Article
Palyzová, Andrea - Šmrhová, T. - Kapinusová, G. - Škrob, Zdena - Uhlík, O. - Řezanka, Tomáš
Stereochemistry of Phosphatidylglycerols from Thermotolerant Bacteria Isolated from Thermal Springs.
Journal of Chromatography A. Roč. 1739, January 4 (2025), č. článku 465517. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA MŠMT(CZ) EH22_008/0004597
Institutional support: RVO:61388971
Keywords : Thermotolerant bacteria * Phosphatidylglycerols * Chiral chromatography * Mass spectrometry * Membrane fluidity * Stereochemistry
OECD category: Analytical chemistry
Impact factor: 3.8, year: 2023 ; AIS: 0.512, rok: 2023
Method of publishing: Limited access
Result website:
https://www.sciencedirect.com/science/article/pii/S0021967324008914?via%3DihubDOI: https://doi.org/10.1016/j.chroma.2024.465517

Phosphatidylglycerol (1,2-diacyl-sn-glycero-3-phospho-glycerol) (PG) is one of the most abundant lipids in biological membranes. However, the chirality of the carbon atom in glycerol phosphate differs among the three kingdoms: bacteria, archaea, and eukaryotes. It is commonly assumed that archaea, as well as bacteria and eukaryotes, produce only one isomer of PG. Archaeal membranes consist of phospholipids with glycerol-1-phosphate in the S configuration, while the phospholipids of the other two kingdoms contain glycerol-3-phosphate with (R) stereochemistry. Another chiral atom is found in glycerol with non-esterified hydroxy groups. Considering the high temperatures that accompanied the origin of life on Earth, it becomes obvious that it is necessary to clarify the importance of membrane lipids in early evolutionary times. To reconstruct the effect of high temperatures on membrane lipids, it is ideal to use microorganisms originating from a thermophilic environment analogous to the early Earth, such as the thermal groundwater of the famous spa town of Karlovy Vary. Here, we prepared all four isomers of PG, i.e., (R,S, R,R, S,R), and (S,S), by organic synthesis and analyzed the representation of individual molecular species in seven bacteria isolated from the Karlovy Vary thermal springs using chiral chromatography - mass spectrometry. Our results provide evidence that five of these strains produce all four isomers of PG and that this production is highly dependent on the cultivation temperature. Subsequent analysis by chiral chromatography revealed that the ratio of isomers, enantiomers, and diastereoisomers depends on the cultivation temperature of individual strains.
Permanent Link: https://hdl.handle.net/11104/0358606