0588514 - ÚPT 2025 RIV US eng J - Článek v odborném periodiku
Uhlířová, Hana - Stibůrek, Miroslav - Pikálek, Tomáš - Gomes, A. - Turtaev, S. - Kolbábková, Petra - Čižmár, Tomáš
“There’s plenty of room at the bottom”: deep brain imaging with holographic endo-microscopy.
Neurophotonics. Roč. 11, S1 (2024), č. článku S11504. ISSN 2329-423X. E-ISSN 2329-4248
Grant CEP: GA MŠMT EF15_003/0000476; GA MŠMT(CZ) EF16_013/0001775
GRANT EU: European Commission(XE) 101082088 - StrokeGATE; European Commission(XE) 101016787 - DEEPER
Výzkumná infrastruktura: Czech-BioImaging II - 90129; Czech-BioImaging III - 90250
Institucionální podpora: RVO:68081731
Klíčová slova: complex media photonics * wavefront shaping * endoscope * multimode fiber * in-vivo imaging * deep brain imaging
Obor OECD: Optics (including laser optics and quantum optics)
Impakt faktor: 4.8, rok: 2023 ; AIS: 1.118, rok: 2023
Způsob publikování: Open access
Web výsledku:
https://www.spiedigitallibrary.org/journals/neurophotonics/volume-11/issue-S1/S11504/Theres-plenty-of-room-at-the-bottom--deep-brain/10.1117/1.NPh.11.S1.S11504.full#_=_DOI: https://doi.org/10.1117/1.NPh.11.S1.S11504

Significance: Over more than 300 years, microscopic imaging keeps providing fundamental insights into the mechanisms of living organisms. Seeing microscopic structures beyond the reach of free-space light-based microscopy, however, requires dissection of the tissue—an intervention seriously disturbing its physiological functions. The hunt for low-invasiveness tools has led a growing community of physicists and engineers into the realm of complex media photonics. One of its activities represents exploiting multimode optical fibers (MMFs) as ultra-thin endoscopic probes. Employing wavefront shaping, these tools only recently facilitated the first peeks at cells and their sub-cellular compartments at the bottom of the mouse brain with the impact of micro-scale tissue damage. Aim: Here, we aim to highlight advances in MMF-based holographic endo-microscopy facilitating microscopic imaging throughout the whole depth of the mouse brain. Approach: We summarize the important technical and methodological prerequisites for stabile high-resolution imaging in vivo. Results: We showcase images of the microscopic building blocks of brain tissue, including neurons, neuronal processes, vessels, intracellular calcium signaling, and red blood cell velocity in individual vessels. Conclusions: This perspective article helps to understand the complexity behind the technology of holographic endo-microscopy, summarizes its recent advances and challenges, and stimulates the mind of the reader for further exploitation of this tool in the neuroscience research. (c) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License.
Trvalý link: https://hdl.handle.net/11104/0355382