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What are the key factors for the detection of peptides using mass spectrometry on boron-doped diamond surfaces?
- 1.0587891 - FZÚ 2025 RIV CH eng J - Článek v odborném periodiku
Aguedo, J. - Vojs, M. - Vrška, M. - Nemčovič, M. - Pakanová, Z. - Aubrechtová Dragounová, Kateřina - Romanyuk, Olexandr - Kromka, Alexander - Varga, M. - Hatala, M. - Marton, M. - Tkáč, J.
What are the key factors for the detection of peptides using mass spectrometry on boron-doped diamond surfaces?
Nanomaterials. Roč. 14, č. 15 (2024), č. článku 1241. E-ISSN 2079-4991
Grant CEP: GA ČR(CZ) GF23-04322L; GA MŠMT(CZ) EH22_008/0004596
Institucionální podpora: RVO:68378271
Klíčová slova: boron-doped diamond * surface termination * peptides * MALDI * mass spectrometry * nanostructures
Obor OECD: Condensed matter physics (including formerly solid state physics, supercond.)
Impakt faktor: 5.3, rok: 2022
Způsob publikování: Open access
We investigated the use of BDD with different surface morphologies for the enhanced detection of nine different peptides by MALDI-MS. For the first time, we compared three different nanostructured BDD film morphologies with differently terminated surfaces to commercially available Ground Steel plates. All these surfaces were evaluated for their effectiveness in detecting the nine different peptides by MALDI-MS. Our results demonstrated that certain nanostructured BDD surfaces exhibited superior performance for the detection of especially hydrophobic peptides, with a limit of detection of down to 2.3 pM. Further investigation showed that hydrophobic peptides were effectively detected on hydrogen-terminated BDD surfaces. On the other hand, the highly acidic negatively charged peptide adrenocorticotropic hormone fragment 18–39 was effectively identified on oxygen-/fluorine-terminated BDD surfaces. Furthermore, BDD surfaces reduced sodium adduct contamination significantly.
Trvalý link: https://hdl.handle.net/11104/0354963
Vědecká data: Zenodo
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