0587538 - ÚEM 2025 RIV GB eng J - Článek v odborném periodiku
Fabbri, R. - Scidá, A. - Saracino, E. - Conte, G. - Kovtun, A. - Candini, A. - Kirdajová, Denisa - Spennato, D. - Marchetti, Valeria - Lazzarini, Ch. - Konstantoulaki, A. - Dambruoso, P. - Caprini, M. - Muccini, M. - Ursino, M. - Anděrová, Miroslava - Treossi, E. - Zamboni, R. - Palermo, V. - Benfenati, V.
Graphene oxide electrodes enable electrical stimulation of distinct calcium signalling in brain astrocytes.
Nature Nanotechnology. Roč. 19, č. 9 (2024), s. 1344-1353. ISSN 1748-3387. E-ISSN 1748-3395
Grant CEP: GA ČR(CZ) GA20-05770S; GA MŠMT(CZ) LM2023050
GRANT EU: European Commission(XE) 956325 - TUBE
Institucionální podpora: RVO:68378041
Klíčová slova: TRPA1 channels * TRPV4 * responses * expression * membrane * endfeet * release * synapse
Obor OECD: Neurosciences (including psychophysiology
Impakt faktor: 38.1, rok: 2023 ; AIS: 13.244, rok: 2023
Způsob publikování: Open access
Web výsledku:
https://www.nature.com/articles/s41565-024-01711-4DOI: https://doi.org/10.1038/s41565-024-01711-4

Astrocytes are responsible for maintaining homoeostasis and cognitive functions through calcium signalling, a process that is altered in brain diseases. Current bioelectronic tools are designed to study neurons and are not suitable for controlling calcium signals in astrocytes. Here, we show that electrical stimulation of astrocytes using electrodes coated with graphene oxide and reduced graphene oxide induces respectively a slow response to calcium, mediated by external calcium influx, and a sharp one, exclusively due to calcium release from intracellular stores. Our results suggest that the different conductivities of the substrate influence the electric field at the cell–electrolyte or cell–material interfaces, favouring different signalling events in vitro and ex vivo. Patch-clamp, voltage-sensitive dye and calcium imaging data support the proposed model. In summary, we provide evidence of a simple tool to selectively control distinct calcium signals in brain astrocytes for straightforward investigations in neuroscience and bioelectronic medicine.
Trvalý link: https://hdl.handle.net/11104/0354672